Agent X

First Appearance

Quack Pack (1996)

Voice

Kath Soucie

Chef Skinner

First Appearance 
Ratatouille (2007)

Voice

Ian Holm

Mitch, aka Big Mitch

 First Appearance

Phineas and Ferb (2007)

Voice

David Mitchell

AUTO

First Appearance 
WALL·E (2008)

Voice

MacInTalk

Steelbeak

First Appearance

Darkwing Duck (1991)

Voice

Rob Paulsen

Chameleon

First Appearance

Mighty Ducks: The Animated Series (1996)

Voice

Frank Welker

Kron

First Appearance

 Dinosaur (2000)

Voice

Samuel E. Wright 

Phantasos

First Appearance

Hercules - TV Series (1998)

Origin
Greek myth

Voice

Tom Kenny

Dijon

First Appearance

DuckTales - The Movie: Treasures of the Lost Lamp (1990)

Other Appearances

DuckTales (1987)

(for clarification: the episodes first aired in 1990.)

Voice

Richard Libertini

Judge Doom

First Appearance

Who Framed Roger Rabbit (1988)

Performed by

 Christopher Lloyd

Maleficent's Goons

First Appearance

Sleeping Beauty (1959)

Other Appearances

Who Framed Roger Rabbit (1988)
House of Mouse (2001)

Mickey's House of Villains (2001)

Voices
 Candy Candido
Pinto Colvig
Dallas McKennon

Original Design
Bill Peet

Original Animator

John Lounsbery

Shan-Yu

First Appearance

Mulan (1998)

Other Appearances

House of Mouse (2001)

Voice
Miguel Ferrer

Original Animators

Pres Romanillos

Tony Stanley

Travis Blaise

Frank R. Cordero

Tom DeRosier

Trey Finney

Anthony Ho Wong

Lotso

First Appearance

Toy Story 3 (2010)

Voice

Ned Beatty

Fox

First Appearance

Gargoyles (1994)

Other Appearances

Gargoyles: The Goliath Chronicles (1996)

Voice

Laura San Giacomo

Si and Am

First Appearance

Lady and the Tramp (1955)

Other Appearances

Lady and the Tramp II: Scamp's Big Adventure (2001)
House of Mouse (2001)
Mickey's House of Villains (2001)

Voices

Peggy Lee

Tress MacNeille

Mary Kay Bergman (Si only)

Scar

First Appearance

The Lion King (1994)

Other Appearances

Timon & Pumbaa (1995)
The Lion King II: Simba's Pride (1998)
House of Mouse (2001)
The Lion King 1½ (2004)

Voice
Jeremy Irons
Jim Cummings

Original Animators

Andreas Deja

Doug Frankel

Nancy Kniep

Mark Koetsier

Jean Morel

Martin Schwartz

Marianne Tucker

Alexander Williams

Kathleen M. Bailey

Jamie Kezlarian Bolio

Diana Coco

Poe DeSpell

First Animated Appearance

DuckTales (1987)

Origin

Uncle Scrooge comic books by Carl Barks

Voice

Frank Welker
Terrence McGovern

Rumplewatt the Giant

First Appearance

Giantland (1933)

Origin

English folklore

Rat Capone

First Appearance

Chip 'n' Dale: Rescue Rangers (1989)

Voice

Jim Cummings

Amin Damoola

First Appearance

Aladdin - TV Series (1994)

Voice

Jeff Bennett

Mor'du

 First Appearance

Brave (2012)

Other Appearances

The Legend of Mor'du (2012)

Chick Hicks

First Appearance

Cars  (2006)

Voice

Michael Keaton

Commander Lyle Tiberius Rourke

First Appearance

Atlantis: The Lost Empire (2001)

Other Appearances

House of Mouse (2001)

Voice
James Garner

Original Animators

Michael Surrey
Chris Wahl
Danny Galieote
Mark
Koetsier

The Satyr, from The China Shop

First Appearance

The China Shop (1934)

Mirage, from The Incredibles

First Appearance

The Incredibles (2004)

Voice

 Elizabeth Peña

The Spider, from The Flying Mouse

First Appearance

The Flying Mouse (1934)

Queen La

First Appearance

The Legend of Tarzan (2001)

Voice

Diahann Carroll

Dr. Jacques Von Hämsterviel

First Appearance

Lilo & Stitch (2002) (cameo)

Other Appearances

Stitch! The Movie (2003)

Lilo & Stitch: The Series (2003)
Leroy & Stitch (2006)

Stitch! (2008)

Voice

Jeff Bennett

Hiroshi Yanaka

Kirk Thornton

Kaa

First Appearance

The Jungle Book (1967)

Origin
The Jungle Books by Rudyard Kipling

Other Appearances

Jungle Cubs (1996)

House of Mouse (2001)
Mickey's House of Villains (2001)
The Jungle Book 2 (2003)

Voice

Sterling Holloway

Jim Cummings

Original Animators

Frank Thomas

Milt Kahl

Gravitina

 First Appearance

Buzz Lightyear of Star Command (2000)

Voice

Kerri Kenney